LOCUS       40924_48363        3422 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pZero++ Amp, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3422)
  AUTHORS   Wu G, Zhu Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-MAR-2004) Fish Genetics, Institute of Hydrobiology, 
            Chinese Academy of Science, 7# South Road of East Lake, Wuhan, Hubei
            430072, People's Republic of China
REFERENCE   2  (bases 1 to 3422)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 3422)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (09-MAR-2004) Fish Genetics, Institute of Hydrobiology, Chinese 
            Academy of Science, 7# South Road of East Lake, Wuhan, Hubei 430072,
            People's Republic of China"
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3422
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    107..128
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        143..173
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    181..197
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     205..221
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        239..257
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     promoter        complement(449..467)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(474..490)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             628..927
                     /codon_start=1
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /translation="QFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKV
                     SRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     rep_origin      955..1334
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     CDS             complement(1385..2242)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      2653..3241
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
       61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
      121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
      181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagctat
      241 ttaggtgaca ctatagaata ctcaagctat gcatcagatc tcatatgcct aggagctccg
      301 cggacgtcga caagcttggt accaagtatg acatggcgcc atgggagtct tggggatcca
      361 ctagtaacgg ccgccagtgt gctggaattc tgcagatatc catcacactg gcggccgctc
      421 gagcatgcat ctagagggcc caattcgccc tatagtgagt cgtattacaa ttcactggcc
      481 gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca
      541 gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc
      601 caacagttgc gcagcctata cgtacggcag tttaaggttt acacctataa aagagagagc
      661 cgttatcgtc tgtttgtgga tgtacagagt gatattattg acacgccggg gcgacggatg
      721 gtgatccccc tggccagtgc acgtctgctg tcagataaag tctcccgtga actttacccg
      781 gtggtgcata tcggggatga aagctggcgc atgatgacca ccgatatggc cagtgtgccg
      841 gtctccgtta tcggggaaga agtggctgat ctcagccacc gcgaaaatga catcaaaaac
      901 gccattaacc tgatgttctg gggaatataa atgtcaggcc tgaatggcga atggacgcgc
      961 cctgtagcgg cgcattaagc gcgcgggtgt ggtggttacg cgcagcgtga ccgctacact
     1021 tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc
     1081 cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagagcttt
     1141 acggcacctc gaccgcaaaa aacttgattt gggtgatggt tcacgtagtg ggccatcgcc
     1201 ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt
     1261 gttccaaact ggaacaacac tcaaccctat cgcggtctat tcttttgatt tataagggat
     1321 gttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat tttaacaaaa
     1381 tttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
     1441 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
     1501 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
     1561 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
     1621 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
     1681 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
     1741 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
     1801 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
     1861 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
     1921 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
     1981 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc
     2041 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
     2101 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
     2161 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
     2221 cacggaaatg ttgaatactc atgcgaaacg atcctcatcc tgtctcttga tcagatcctg
     2281 atcccctgcg ccatcagtcc ttggcggcga gaaagccatc cagtttactt tgcagggctt
     2341 cccaacctta ccagagggcg ccccagctgg caattccggt tcgcttgctg tccataaaac
     2401 cgcccagtct agctatcgcc atgtaagccc actgcaagct acctgctttc tctttgcgct
     2461 tgcgttttcc cttgtccaga tagcccagta gctgacattc atccggggtc agcaccgttt
     2521 ctgcggactg gctttctacg tgaaaaggat ctaggtgaag atcctttttg ataatctcat
     2581 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
     2641 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     2701 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     2761 ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt
     2821 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     2881 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     2941 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     3001 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     3061 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     3121 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     3181 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     3241 aaacgccagc aacgcggcct ttttacggtt cctgggcttt tgctggcctt ttgctcacat
     3301 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
     3361 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga
     3421 ag
//
